Description

PNPG (Gus);4-Nitrophenyl-β-D-glucuronic acid;p-Nitrophenyl-β-D-glucuronide; PNP-β-D-GlcAMOLECULAR BIOLOGY GRADEC₁₂H₁₃NO₉MW: 315.240 g/molSlightly off-white to yellow crystalline powder.PNPG (Gus) is a chromogenic β-glucuronidase substrate, yielding a yellow solution upon cleavage. It is used to detect E. coli contaminationin food and drinking water due to their β-glucuronidase activity.Storage/Handling: Store desiccated at -20°C, and protect from light.References:1. M. Kilian, P. BŸlow, Rapid diagnosis of Enterobacteriacea e. I. Detection of bacterial glycosidases. Acta Pathol. Microbiol. Scand. Sect. B 1976, 84, 245. 2. L. Le Minor, Te trathionate reductase, beta-glucuronidase, and ONPG-test in the genus Salmonella. Zbl. Bakt. Hyg. I. Abt. Orig. A 1 979, 243, 321.3. M. Kilian, P. BŸlow, Rapid identification of Enterobacteriaceae. II. Use of a beta-glucuronidase detecting agar medium (PGUA agar) for the identification of E. coli in primary cultures of urine samples. Acta. Pat h. Microbiol. Scand. Sect. B 1979, 87, 271.4. M. Manafi, et al., Fluorogenic and chromogenic substrates used in bacteri al diagnostics. Microbiol. Rev. 1991, 55, 335. 5. I.D. Ogden, A.J. Watt, An evaluation of fluorogenic and chromogen ic assays for the direct enumeration of Escherichia col i. Lett. Appl. Microbiol. 1991, 13, 212.6. R.H. Nimmo-Smith , p-Nitrophenyl-beta-glucuronide as substrate for beta-glucu ronidase. Biochim. Biophys. Acta 1961, 50, 166.