请使用支持JavaScript的浏览器!
生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
banner
当前位置: 首页 > 产品中心 > Protein Expression & Purification > GoldBio/TMB, free base/25 g/TMBF25

GoldBio/TMB, free base/25 g/TMBF25

GoldBio/TMB, free base/25 g/TMBF25
  • GoldBio/TMB, free base/25 g/TMBF25
商品介绍

3,3’,5,5’-Tetramethylbenzidine, free baseTMBMOLECULAR BIOLOGY GRADEC16H20N2MW: 240.35 g/molTMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.Storage/Handling: Store desiccated at 4°C. Protect from light.PubChem CID: 41206

品牌介绍

About GoldBio:

GoldBio Research

Gold Biotechnology shares a vision with every researcher: Each facet of life science research will introduce us to a future of impactful discovery and learning, benefiting the entire world. And our vision will materialize sooner if a researcher’s passion is not sullied by worry over the quality of their reagents, especially the cost for quality. This is why we’re here! We believe in a future of change and prosperity, and we want to help you get there by being a resource for high-quality products at exceptional prices. By providing our customers with only the best chemicals possible, tested in GoldBio labs to assure our own quality specifications are met, you can be sure your research will go off without a hitch.

Mission Statement:

"At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all."

Our Philosophy:

Provide the highest quality product
Maintain low costs to pass along to the researcher
Be an advocate for the industry
Support our customers as any loyal business partner
Be an ally to businesses in the industry
Put forth products with hope
Look towards the future
We look toward the future with hope as we send out our products with the intent that they will be used in great discovery. We are a small company dedicated to the success of those whom we supply. So we provide high-quality low cost materials to scientists and researchers. We give our customers the best so they can discover, invent, and innovate. Because that is what it is about for us...The Discovery.

Commitment to Quality:

We are dedicated to providing our customers with the highest-quality products available. We test our products from our network of trusted global suppliers to assure they meet our quality specifications. We stand behind all of our products knowing that our reputation lies on the success of your experiments.

History:

Dr. Paul Gold

During the 1980s, Dr. Paul Gold stepped out from behind the bench believing researchers needed a low-cost, high-quality alternative for chemicals and reagents. Recognizing his knack for business, he began brewing the idea for a company during a time when startup incubators were nearly unheard of, and the Internet, social media and crowdfunding didn’t exist. Armed with a supportive wife, generous family members and helpful people within his network, Dr. Gold established GoldBio in 1986.

The original main office was situated in the former St. Louis Technology Center (now the location for the St. Louis Science Center), while the first lab space was located at St. Louis University. The ambitious startup began its journey selling three products: IPTG, X-Gal and X-Gluc.

Now, after almost 30 years, GoldBio has evolved significantly, expanding its catalog from three products to more than 3000 products. Throughout its history, GoldBio’s success has relied on its commitment to the success of those whom we supply. At GoldBio, we believe that putting our quality products in capable hands leads to discoveries that impact the entire world。

 


苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


材料
l  D-荧光素盐
u  荧光素钾盐(启维益成, G156410
u  荧光素钠盐(启维益成,G156411
l  DPBS(Dulbecco’s phosphate-buffered saline), without Ca2+ 或 Mg2+
l  Syringe filter, 0.2um
 

荧光素制备
1.       室温下融解D-荧光素(钾盐或钠盐)溶于DPBS(不含钙或镁)配成终浓度为15mg/ml。
2.       用5-10ml灭菌水预湿0.22 um滤膜然后弃去灭菌水。
3.       通过准备好的0.22 um注射器式滤膜灭菌荧光素溶液。
4.       按照不同的方法接种到实验室动物中(通常荧光素通过腹腔注射或静脉注射的方式接种到动物体内)。
比如:按10ul荧光素储存液/g体重的比例来注射(正常情况下每20g小鼠注射200ul,标准150mg/kg注射)。
5.       成像之前等待10-20分钟可达到最大荧光素酶信号稳定期。
6.       完成每个动物模型的荧光素酶动力学研究,以确定其信号达峰时间和平台期。
 
确定动力学曲线
  每个动物模型和实验设计的组织生物分布的动力学是不同的,动物模型注射完荧光素后为了确定达峰信号时间,在实验之前建议先建立每个体系的动力学曲线。
为您的动物模型的荧光素酶活性建立动力学曲线:
 
1.       通过以下推荐的每个所列方法(腹腔注射,静脉注射或皮下注射)注射荧光素。注射之前如果需要给动物镇静,注意这样可能使动力学曲线(荧光素酶达峰时间)稍稍延伸。根据给药途径不同荧光素的生物分布是不同的。
2.       对于腹腔(IP)或皮下(SQ)注射:
   a.       如果你要注射荧光素时动物依旧是清醒的,等待三分钟,然后根据您的方法选择(气体或注射麻醉)使其镇静。参看附录关于大鼠和小鼠麻醉剂/镇痛药物和剂量的使用。
   b.       把已经使用镇静剂的动物放入成像室中,在注射荧光素后大约五分钟能看到荧光成像。
3.       对于静脉注射(IV):
   a.       立即对动物进行镇静(如果没有镇静)根据您的方法来选择(气体或注射麻醉)使其镇静。参看附录关于大鼠和小鼠麻醉剂/镇痛药物和剂量的使用。
   b.       把已经使用镇静剂的动物放入成像室中,在注射荧光素后两分钟内能看到荧光成像。
4.       为建立荧光素酶表达的动力学曲线,对于腹腔(IP)或皮下(SQ)注射方法每隔5-10分钟拍摄一次成像时间可达40-60 分钟(对于静脉注射(IV)方法:每1-5分钟拍摄一次可达20-30分钟)。(大部分注射麻醉剂可持续20-30分钟,对于完整动力学曲线研究将需要额外的剂量!)
5.       对于您的动物模型从动力学曲线中选择最佳的成像时间点。大部分动物模型对于腹腔注射或皮下注射荧光素注入动物体内后大约10-20分钟达到信号峰值,对于静脉注射荧光素没注入后2-5分钟达到峰值。
自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496