Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.
NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.
NBT Reduction Test: Assessment of reduction of NBT dye by stimulated and unstimulated neutrophils.
Product SpecificationsNitrotetrazolium blue chloride, NBT;Nitro blue tetrazolium chloride;Nitro-TB
MOLECULAR BIOLOGY GRADE
Formula: C40H30N10O6Cl2
MW: 817.65 g/mol
Storage/Handling: Store at 4°C. Protect from light.
PubChem Chemical ID: 9281
IPTG, X-Gal and X-Gluc
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没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal
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