DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3" position and a phosphate group at the 5" position. The optimum pH is 7.8.
DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.Product SpecificationsDNase IDeoxyribonuclease IDNase I, Bovine Pancreas >2000 Kunitz U/mg
Unit Definition: One Kunitz Unit will produce an A260 of 0.001 per minute at pH 5.0 at 25°Cusing DNA, type I or III as the substrate.
MOLECULAR BIOLOGY GRADE
Storage/Handling: Store desiccated at -20°C.Allow product to come to room temperature before opening.
For greatest stability, it is important that DNase to be dissolved at a concentration of at least 1 mg/ml in 50% glycerol with 20mM Tris-HCl, pH 7.5, and 1mM MgCl2.
IPTG, X-Gal and X-Gluc
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没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal
本网站将在规定时间内给予删除等相关处理。
