Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc).It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Product Specifications:Coelenterazine 400 a2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one DeepBlueCTM
Formula: C26H21N3O
MW: 391.48 g/mol
Purity: >95% by TLC
Color: Yellow to orange powder
Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.Soluble in methanol or ethanol (NOT in DMSO). Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that willspontaneously decompose even at low temperatures.
PubChem CID:135750016
IPTG, X-Gal and X-Gluc
©蚂蚁淘
没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal