5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeastmolecular genetics. The toxicity of 5-FOA to yeast cells with afunctioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMPdecarboxylase), makes it especially useful for the selection andidentification of mutant yeast strains.5-FOA is most often appliedwith Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli(pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for theconstruction of activation domain hybrid libraries to identifyprotein-protein interactions.
Product Specifications:5-Fluoroorotic acid monohydrate (5-FOA)5-Fluorouracil-4-carboxylic acid
MOLECULAR BIOLOGY GRADE
Formula: C5H3FN2O4 • H2O
MW: 192.10 g/mol
White to slightly off-white crystalline powder
Storage/Handling: Store at -20°C. Protect from light.
PubChem Chemical ID: 16212749
IPTG, X-Gal and X-Gluc
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没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal