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生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
℡ 4000-520-616
℡ 4000-520-616
GoldBio/Goof-Proof™ qPCR Master Mix with ROX/5 x 1 mL/G-705-5
产品编号:G-705-5
市  场 价:¥4320.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>其他试剂>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$216.00
品      牌: GoldBio
公      司:Gold Biotechnology, Inc.
公司分类:
GoldBio/Goof-Proof™ qPCR Master Mix with ROX/5 x 1 mL/G-705-5
商品介绍

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

Figure1: 1x Goof-Proof™Master MixFigure 2: 1x Goof-Proof™ Master Mix after adding 1X Goof-Proof™ Template Buffer

Kit components

  • Goof-Proof™ 2X Master Mix -The 2X Master Mix contains GoldBio HotStart Taq DNA Polymerase, dNTPs and a low concentration of an inert blue dye. The dye helps the user distinguish between empty wells and wells containing master mix.
  • Goof-Proof™ Template Buffer -40X Template Buffer contains a high concentration of inert blue dye. Adding the template buffer to the reaction mix makes it "Goof-Proof" to track which wells have had template added to the PCR reactions. Template Buffer is not required for the Master Mix to function, but is designed to help alleviate mistakes with template addition.
  • ROX Reference Dye -Proof™ qPCR Master Mix with ROX includes a vial of ROX reference dye. ROX is necessary for accurate Ct determination from well to well with certain instruments (such as some ABI and Stratagene PCR instruments, refer to your instrument instructions to see if ROX is required). ROX may add noise to melt curve analysis, which could be mistaken for real peaks. In the case of unexpected peaks, un-check “ROX” in the “Passive Reference Dye” box in the system software to prevent data collection from the ROX fluorescence channel and re-analyze the data.

Note: We also offer Goof-Proof™ qPCR Master Mix (Catalog # G-700) without ROX for instruments that do not require the ROX reference dye.

Storage/Handling: Store the kit at -20°C.

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

品牌介绍

IPTG, X-Gal and X-Gluc

©蚂蚁淘

没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:

琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

培养基添加剂

洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal

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