GoldBio"s Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates.
Our RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Product Includes
- Reverse Transcriptase
- 10x RT Reaction Buffer
1X MMLV Reverse Transcriptase Reaction Buffer
- 50mM Tris-HCl, 75mM KCl, 3mM MgCl2, 10mM DTT, pH 8.3 at 25°C
Storage Buffer
- 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 at 25ºC
Source: The gene encoding recombinant MMLV Reverse Transcriptase with mutated RNase H domain is expressed in E. coli.
Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Heat Inactivation: 65°C for 20 minutes.
Storage/Handling: Store at -20°C. Product may be shipped on blue ice.
IPTG, X-Gal and X-Gluc
©蚂蚁淘
没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal
本网站将在规定时间内给予删除等相关处理。
