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生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
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GoldBio/SHH, Human/500 μg/6110-19-500

GoldBio/SHH, Human/500 μg/6110-19-500
  • GoldBio/SHH, Human/500 μg/6110-19-500
商品介绍

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Sonic HedgeHogrHuShhHhg2

Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related and share a high degree of amino acid sequence identity. Each Hh protein has a unique expression pattern and distinct biological role within its respective region of secretion.All Hh proteins use the same signaling pathway and can substitute for each other in experimental systems. Shh has been found to have a critical role in development, acting as a morphogen in the patterning of limbs, brain structure, spinal cord, thalamus and the teeth.Shh has been shown to attract commissural axons in the developing spinal cord by attracting or repelling retinal ganglion cell (RGC) axons depending on concentration.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: IVIGPGRGFGKRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQRCKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFDWVYYESKAHI HCSVKAENSV AAKSGG

MW: ~20.0 kDa (176 amino acid)

Purity: >98 % bySDS-PAGE and HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than1.0 μg/ml, corresponding to a specific activity of >1.0 × 103 IU/mg.

Accession Number: Q15465

HGNC ID: 10848

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80°C for up to 6 months.Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!

品牌介绍

About GoldBio:

GoldBio Research

Gold Biotechnology shares a vision with every researcher: Each facet of life science research will introduce us to a future of impactful discovery and learning, benefiting the entire world. And our vision will materialize sooner if a researcher’s passion is not sullied by worry over the quality of their reagents, especially the cost for quality. This is why we’re here! We believe in a future of change and prosperity, and we want to help you get there by being a resource for high-quality products at exceptional prices. By providing our customers with only the best chemicals possible, tested in GoldBio labs to assure our own quality specifications are met, you can be sure your research will go off without a hitch.

Mission Statement:

"At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all."

Our Philosophy:

Provide the highest quality product
Maintain low costs to pass along to the researcher
Be an advocate for the industry
Support our customers as any loyal business partner
Be an ally to businesses in the industry
Put forth products with hope
Look towards the future
We look toward the future with hope as we send out our products with the intent that they will be used in great discovery. We are a small company dedicated to the success of those whom we supply. So we provide high-quality low cost materials to scientists and researchers. We give our customers the best so they can discover, invent, and innovate. Because that is what it is about for us...The Discovery.

Commitment to Quality:

We are dedicated to providing our customers with the highest-quality products available. We test our products from our network of trusted global suppliers to assure they meet our quality specifications. We stand behind all of our products knowing that our reputation lies on the success of your experiments.

History:

Dr. Paul Gold

During the 1980s, Dr. Paul Gold stepped out from behind the bench believing researchers needed a low-cost, high-quality alternative for chemicals and reagents. Recognizing his knack for business, he began brewing the idea for a company during a time when startup incubators were nearly unheard of, and the Internet, social media and crowdfunding didn’t exist. Armed with a supportive wife, generous family members and helpful people within his network, Dr. Gold established GoldBio in 1986.

The original main office was situated in the former St. Louis Technology Center (now the location for the St. Louis Science Center), while the first lab space was located at St. Louis University. The ambitious startup began its journey selling three products: IPTG, X-Gal and X-Gluc.

Now, after almost 30 years, GoldBio has evolved significantly, expanding its catalog from three products to more than 3000 products. Throughout its history, GoldBio’s success has relied on its commitment to the success of those whom we supply. At GoldBio, we believe that putting our quality products in capable hands leads to discoveries that impact the entire world。

 


蚂蚁淘生物致力于成为科研试剂搜索、采购一体化解决方案提供商!

我们的优势: 

1,覆盖欧美的全球采购中心,总部设立在美国新泽西,采购团队有30多人,能进口涵盖抗体,蛋白,细胞,血清,耗材等的全门类生物产品。

2,货品多且全,目前,以我们美国公司为主体,与欧美600多个品牌建立了正式的代理与合作,基本涵盖所有的生物试剂门类,特别是二三线高质量品牌产品,优势更大! 



活体成像两种检测技术介绍

活体成像

特点

优点

缺点

生物发光检测

bioluminescence

★荧光素酶(Luciferase)对基因、细胞和活体动物进行标记;

★荧光素酶催化底物(例如荧光素钾盐)反应后,会产生化学发光。这种光是由化学反应而来,不需要激发光;

★标记方法是通过克隆技术,将荧光素酶的基因插入到预期观察的细胞染色体内,通过对克隆细胞进行筛选,培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。

★特异性强,无自发荧光;

★高灵敏度,在体内可检测到几百个细胞,检测的深度在3-100px;

★定量精确

 

★信号较弱,检测时间较长;★仪器精密度要求较高;

★细胞或基因需要转基因标记;

★不可用于人体,不适用于抗体、多肽等标记

荧光检测

fluorescence

★采用荧光报告基因 (GFP、RFP等) 或荧光染料进行标记;

★需要外接激发光源,利用报告基因、荧光蛋白质或染料产生的荧光,就可以形成体内的生物光源。

★荧光染料、蛋白标记能力强;

★信号强,成像速度快,操作简便,实验成本较低;

★未来可用于人;

★适用范围广,可以是动物、细胞、微生物,也可以是抗体、药物、纳米材料等。

★存在自发荧光,影响灵敏度;

★光容易被动物组织吸收;

★检测深度受限;

★背景光干扰,定量准确度低


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