GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Murine Colony Stimulating Factor 1 (Macrophage)Macrophage Colony Stimulating Factor, M-CSF

RecombinantMurine Colony Stimulating Factor 1 (Macrophage) (CSF1), formerly called Macrophage Colony Stimulating Factor (M-CSF), is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. CSF1 is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for CSF1 gene. Although forms may vary, all of them contain the N-terminal 150 amino acid portion that is necessary and sufficient for interaction with the receptor. The first 229 amino acids of mature mouse CSF1 shares 87%, 83%, 82% and 81% sequence identity with corresponding regions of rat, dog, cow and human CSF1, respectively. Human CSF1 is active in the mouse, but mouse CSF1 is reported to be species-specific.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: KEVSEHCSHMIGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERLQELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRDVVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQRPPRSTCQTLE

MW: ~52.0 kDa (a disulfide-linked homodimer consisting of two 230amino acid polypeptide chains).

Purity: >95% by SDS-PAGEand HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine M-NFS-60 cells is less than2.0 ng/ml, corresponding to a specific activity of >5.0 × 10⁹ IU/mg.

Accession Number: Q3U4F9

MGI ID: 1339753

Source: Escherichia coli

Storage/Handling: This lyophilized preparationis stable at 2-8°C, but should be kept at -20°C for long term storage. Thereconstituted sample can be apportioned into working aliquots and stored at-80°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions containing BSA or serum.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!

IPTG, X-Gal and X-Gluc

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琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

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洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal