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生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
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GoldBio/DEFB3, Rat/20 μg/1590-03-20

GoldBio/DEFB3, Rat/20 μg/1590-03-20
  • GoldBio/DEFB3, Rat/20 μg/1590-03-20
商品介绍

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

RecombinantRat Defensin,Beta 3BD3, DEFB-3, RBD-3, DEFB103A

Rat beta-defensin 3 (DEFB3) is an antimicrobial peptide that contributes to the innate and adaptive immune systems and is active against gram-negative and gram-positive bacteria, fungi, and viruses. Like the other β-defensins, DEFB3 is a small protein that contains a motif consisting of six cysteine residues which form three intramolecular disulfide bridges. It is expressed in epithelial tissue of many organs, including the skin and lungs, and in leukocytes. Expression of DEFB3 is induced by proinflammatory cytokines such as IL1B and IFNG. Expression of rat DEFB3 was also found to be induced in bronchoalveolar lavage fluid by inflammatory diesel exhaust particles. DEFB3 is a cationic peptide that disrupts the membranes of invading microbes, which are negatively charged due to the presence of lipopolysaccharides (LPS) or lipoteichoic acid (LTA). Inhibition of DEFB3 by high salt concentration may play a role in the pathogenesis of cystic fibrosis.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Source: Escherichia coli

MW: ~4.5 kDa (41 aminoacids)

Purity: >95% by SDS-PAGEand HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20 μg/ml.

Storage/Handling: Store desiccated at-20°C.

RGD: 1589572

NOT FOR HUMAN USE!

Additional resources for this protein may be found at:OMIM

品牌介绍

About GoldBio:

GoldBio Research

Gold Biotechnology shares a vision with every researcher: Each facet of life science research will introduce us to a future of impactful discovery and learning, benefiting the entire world. And our vision will materialize sooner if a researcher’s passion is not sullied by worry over the quality of their reagents, especially the cost for quality. This is why we’re here! We believe in a future of change and prosperity, and we want to help you get there by being a resource for high-quality products at exceptional prices. By providing our customers with only the best chemicals possible, tested in GoldBio labs to assure our own quality specifications are met, you can be sure your research will go off without a hitch.

Mission Statement:

"At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all."

Our Philosophy:

Provide the highest quality product
Maintain low costs to pass along to the researcher
Be an advocate for the industry
Support our customers as any loyal business partner
Be an ally to businesses in the industry
Put forth products with hope
Look towards the future
We look toward the future with hope as we send out our products with the intent that they will be used in great discovery. We are a small company dedicated to the success of those whom we supply. So we provide high-quality low cost materials to scientists and researchers. We give our customers the best so they can discover, invent, and innovate. Because that is what it is about for us...The Discovery.

Commitment to Quality:

We are dedicated to providing our customers with the highest-quality products available. We test our products from our network of trusted global suppliers to assure they meet our quality specifications. We stand behind all of our products knowing that our reputation lies on the success of your experiments.

History:

Dr. Paul Gold

During the 1980s, Dr. Paul Gold stepped out from behind the bench believing researchers needed a low-cost, high-quality alternative for chemicals and reagents. Recognizing his knack for business, he began brewing the idea for a company during a time when startup incubators were nearly unheard of, and the Internet, social media and crowdfunding didn’t exist. Armed with a supportive wife, generous family members and helpful people within his network, Dr. Gold established GoldBio in 1986.

The original main office was situated in the former St. Louis Technology Center (now the location for the St. Louis Science Center), while the first lab space was located at St. Louis University. The ambitious startup began its journey selling three products: IPTG, X-Gal and X-Gluc.

Now, after almost 30 years, GoldBio has evolved significantly, expanding its catalog from three products to more than 3000 products. Throughout its history, GoldBio’s success has relied on its commitment to the success of those whom we supply. At GoldBio, we believe that putting our quality products in capable hands leads to discoveries that impact the entire world。

 


苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司,以常规生物学实验外包与生物学试剂为基础,放眼于生命科学领域的前沿技术服务,公司技术骨干有着多年的生物学科研背景,毕业于国内外名牌大学,并且有着丰富的项目经验,了解国内外科研行情,我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点,和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括:抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人,认真做事,服务至上“,希望用我们的专业知识和项目经验为国内科研客户解决科研问题,祝您科研之路畅通无阻!


活体成像两种检测技术介绍

活体成像

特点

优点

缺点

生物发光检测

bioluminescence

★荧光素酶(Luciferase)对基因、细胞和活体动物进行标记;

★荧光素酶催化底物(例如荧光素钾盐)反应后,会产生化学发光。这种光是由化学反应而来,不需要激发光;

★标记方法是通过克隆技术,将荧光素酶的基因插入到预期观察的细胞染色体内,通过对克隆细胞进行筛选,培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。

★特异性强,无自发荧光;

★高灵敏度,在体内可检测到几百个细胞,检测的深度在3-100px;

★定量精确

 

★信号较弱,检测时间较长;★仪器精密度要求较高;

★细胞或基因需要转基因标记;

★不可用于人体,不适用于抗体、多肽等标记

荧光检测

fluorescence

★采用荧光报告基因 (GFP、RFP等) 或荧光染料进行标记;

★需要外接激发光源,利用报告基因、荧光蛋白质或染料产生的荧光,就可以形成体内的生物光源。

★荧光染料、蛋白标记能力强;

★信号强,成像速度快,操作简便,实验成本较低;

★未来可用于人;

★适用范围广,可以是动物、细胞、微生物,也可以是抗体、药物、纳米材料等。

★存在自发荧光,影响灵敏度;

★光容易被动物组织吸收;

★检测深度受限;

★背景光干扰,定量准确度低


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