GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Murine NOGNoggin
Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β (Transforming Growth Factor) family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has been shown to modulate the activities of other BMPs including BMP2, 7, 13 and 14. Targeted deletion of noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Transgenic mice overexpressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture
Sequence: MQHYLHIRPAPSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQLLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVGSCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
MW: ~46.4 kDa (2x206 amino acid)(A non-disulfide linked homodimer consistingof two 206 amino acid polypeptide chains)
Purity: >95% by SDS-PAGEand HPLC analyses
Biological Activity: Fully biologically active compared to standard. The ED50 as determined by inhibiting BMP-4 induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0×105 IU/mg in the presence of 5 ng/ml BMP-4.
Accession Number:P97466
MGI ID: 104327
Source: Escherichia coli
Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in aqueous buffer containing 10mM HAc to a concentration of<0.25 mg/ml. Reconstituted solutions are stable for up to one week at2-8°C.Stock solutions should bealiquoted and stored at -80°C. Further dilutions should be made in appropriatebuffered solutions
Additional resources for this product may be found at:
OMIM
NOT FOR HUMAN USE!
IPTG, X-Gal and X-Gluc
©蚂蚁淘
没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal