DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovinepancreatic deoxyribonuclease (DNase) is an endonuclease which splitsphosphodiester linkages, preferentially adjacent to a pyrimidinenucleotide yielding polynucleotides with a free hydroxyl group at the 3"position and phosphate group at the 5" position. The optimum pH is 7.8.

DNaseI is activated by bivalent metals, and inhibited by chelating agentssuch as EDTA and SDS. DNase I can be stabilized against proteolyticdigestion by 5mM Ca²⁺.

Goldbio DNase is tested on bacterial lysates rather thanon pure DNA. Tests on pure DNA are not as rigorous andtend to be biased towardsuccess.

Product SpecificationsDNase IDeoxyribonuclease IDNase I, Bovine Pancreas, >300 Kunitz U/mg

MOLECULAR BIOLOGY GRADE

Unit Definition: One Kunitz Unit will produce an A₂₆₀ of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.

Storage/Handling: Store desiccated at -20°C.Allow product to come to room temperature before opening.

Forgreatest stability, it is important that DNase to be dissolved at aconcentration of at least 1 mg/ml in 50% Glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl₂.

IPTG, X-Gal and X-Gluc

©蚂蚁淘

没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：

琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

培养基添加剂

洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal