Octylglucoside is a nonionic, mild detergent used to isolate membrane proteins in the native state. It can easily be removed during final extractions by dialysis.
Characteristics that contribute to this detergent’s usefulness are its water solubility, chemical structure and small-sized micelles.
Octylglucoside also provides higher resolution of proteins in 2D electrophoresis.
Product SpecificationsOctylglucoside ultra puren-Octyl-β-D- glucopyranoside n-Octyl-beta-D-Glc
MOLECULAR BIOLOGY GRADE
Formula: C14H28O6
MW: 292.38 g/mol
Storage/Handling: Store at -15°C, and keep container tightly sealed and dry. This product is very hygroscopic.
PubChem Chemical ID: 62852
Literature:
- Gould, R. J., Ginsberg, B. H., &Spector, A. A. (1981). Effects of octyl. beta.-glucoside on insulinbinding to solubilized membrane receptors. Biochemistry, 20(24), 6776-6781.
- Aveldaño, M. I., Diaz, R. S., Regueiro, P., & Monreal, J. (1991). Solubilization of myelin membranes by detergents. Journal of neurochemistry, 57(1), 250-257.
- Halldén, G., Andersson, U., Hed, J.,& Johansson, S. G. O. (1989). A new membrane permeabilization methodfor the detection of intracellular antigens by flow cytometry. Journal of immunological methods, 124(1), 103-109.
- Lopez, M. F. (1999). Proteome analysis: I. Gene products are where the biological action is. Journal of Chromatography B: Biomedical Sciences and Applications, 722(1-2), 191-202.
- Jackson, M. L., Schmidt, C. F.,Lichtenberg, D., Litman, B. J., & Albert, A. D. (1982).Solubilization of phosphatidylcholine bilayers by octyl glucoside. Biochemistry, 21(19), 4576-4582.
- Mimms, L. T., Zampighi, G., Nozaki,Y., Tanford, C., & Reynolds, J. A. (1981). Phospholipid vesicleformation and transmembrane protein incorporation using octyl glucoside.Biochemistry, 20(4), 833-840.
- Yoshida, M., Suzuki, A., Yamamoto, H.,Noguchi, S., Mizuno, Y., & Ozawa, E. (1994). Dissociation of thecomplex of dystrophin and its associated proteins into several uniquegroups by n‐octyl β‐D‐glucoside. European journal of biochemistry, 222(3), 1055-1061.
IPTG, X-Gal and X-Gluc
©蚂蚁淘
没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal