Firefly and Renilla Luciferase Assays are two of the leading reporter assays in the measurement of gene function and gene regulation both in vitro as well as in vivo. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg²⁺ to oxyluciferin producing a yellow to greenish light (~560 nm). Renilla luciferase is a monomeric 36 kDa protein isolated from the sea pansy Renilla reniformis that catalyzes coelenterazine with oxygen to produce a blue light (480 nm).

GoldBio’s Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit is a combined luciferase assay allowing the sequential measurement of Firefly and Renilla luciferase activity in the same sample while still retaining the highest degree of sensitivity, reliability and linearity that you want in your assay. Firefly activity is measured first, then the Renilla Luciferase Assay Buffer is added to the sample to simultaneously extinguish all firefly luciferase activity to the level of untransformed cells and begins the Renilla luciferase activity. This great luciferase combo allows the sequential measurement of both luciferase assays in the same sample without additional hassle.

This kit is a flash-type luminescent assay, and requires measurement immediately after adding the detection reagents to the sample. The Enhanced Assay Kit includes our water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well.

Kit components

• 1X Passive Lysis Buffer
• Firefly Luciferase Assay Buffer
• Renilla Luciferase Assay Buffer
• GoldBio D-Luciferin
• GoldBio Enhanced Coelenterazine

Storage/Handling: Store the kit at -80°C (1X Passive Lysis Buffer, Luciferin and Enhanced Coelenterazine may be stored separately at -20°C).

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

IPTG, X-Gal and X-Gluc

©蚂蚁淘

没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：

琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

培养基添加剂

洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal