GoldBio’s Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit contains a proprietary formulation that modifies and stabilizes the enzymatic reaction to produce a long lasting bioluminescent signal by preventing the formation of adenyl-oxyluciferin at the enzyme surface. The Dura-Luc system is a highly sensitive and homogenous luciferase reporter assay that has a signal half-life of three hours for the best quantification of expression in mammalian cell lines, allowing for batch processing and plate to plate comparisons in high-throughput screening experiments.

Firefly Luciferase Assays are one of the leading reporter assays in the world in the measurement of gene function and gene regulation as well as being widely used in pharmaceutical screening. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg²⁺ to oxyluciferin producing a yellow to greenish light (~560 nm).

The Dura-Luc Lyophilized Firefly Kit contains an assay buffer that is provided in a lyophilized form for economical shipping and more convenient storage. A reconstitution buffer is also provided with this kit.

Kit components

• Dura-Luc Firefly Lyophilized Luciferase Assay Buffer
• GoldBio D-Luciferin
• Reconstitution Buffer

Storage/Handling: Store the kit at -20°C (Reconstituted Assay Buffer should be stored at -80°C to retain performance).

Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

IPTG, X-Gal and X-Gluc

©蚂蚁淘

没错！最开始，Gold Biotechnology只生产这三个产品，30年后的今天，Goldbio的产品已经超过3000种，涵盖：

琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

培养基添加剂

洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是：荧光素钾盐，X-gluc， 双丙氨磷，草胺磷 和 X-α-gal