Acriflavine HCl is a compound predominantly used in cellular biology for fluorescence staining. It intercalates between the base pairs of DNA and RNA. Among the acridine dyes, acriflavine has the highest affinity for DNA. Acriflavine stains eukaryotic cells exceptionally well in humic substance environments. It provides a robust experimental consistency and reduces the effect of minor experimental errors. The binding of acriflavine to DNA can be observed from a characteristic red shift in an absorption spectrum. While having a high affinity for DNA, acriflavine is limited to euchromatin for both DNA and RNA.
Acriflavine has also been found to inhibit heterodimeric transcription factors, specifically called hypoxia-inducible factors (HIFs). Studying the hyperactivity of HIFs has become a popular in oncology research, where chemicals like acriflavine are used to disrupt HIF activity of malignant cells. HIFs are activated in hypoxic conditions and promote angiogenesis to alleviate stress. They are normally regulated through proline and asparagine hydroxylation. Acriflavine halts HIF activity, specifically by binding to a subdomain of HIF-1α and HIF-2α.
Product SpecificationsAcriflavine HClEuflavineXanthacridinum
Formula: C27H25ClN6
MW: 468.98 g/mol
Storage/Handling: Store at 4°C.
PubChem Chemical ID: 443101
NOT FOR HUMAN USE!!
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IPTG, X-Gal and X-Gluc
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没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal