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生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
℡ 4000-520-616
℡ 4000-520-616
GoldBio/Immobilized Ficin Agarose Resin/5 mL/I-010-5
产品编号:I-010-5
市  场 价:¥4300.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$215.00
品      牌: GoldBio
公      司:Gold Biotechnology, Inc.
公司分类:
GoldBio/Immobilized Ficin Agarose Resin/5 mL/I-010-5
商品介绍

Ficin is a cysteine protease enzyme which is derived from the latex of fig trees with activity similar to other cysteine protease enzymes including endopeptidases, aminopeptidases, and dipeptidyl peptidases. Ficin cleaves mouse immunoglobulin G molecules (IgG1) in the hinge region. The concentration of cysteine changes how ficin cleaves IgG1. A F(ab’)2 fragment is formed in the presence of 1mM cysteine. The F(ab’)2 fragments are smaller than whole IgG molecules and better penetrate tissue for antigen recognition while avoiding unspecific binding to Fc receptors. In the presence of 10mM cysteine, two 2 Fab fragments are produced instead. F(ab) fragments block background from primary antibody binding, and block endogenous and exposed immunoglobulins and tissues in labeling experiments.

Immobilized Ficin Agarose Resin offers a convenient solution to the production of F(ab’)2 and Fab fragments while eliminating autolysis and protease contamination and the need to remove the enzymes following digestion. Fab and F(ab’)2 can be further separated from whole IgG with either Protein A Agarose Resin (GoldBio Product # P-400) or ion exchange chromatography.

Activity:1-1.5 mg ficin/mlof resin

Support: 6% Cross-linkedAgarose

Storage/Handling: Shipped at ambienttemperature. Upon receipt, store at 4°C, do NOT freeze.

品牌介绍

IPTG, X-Gal and X-Gluc

©蚂蚁淘

没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:

琼脂糖树脂

抗生素

生化试剂

缓冲液

克隆与诱导

培养基添加剂

洗涤剂

DNA蛋白质电泳

酶、抑制剂和底物

生长因子

植物研究

蛋白质表达与纯化

还原剂……

目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal

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