LOW Density Aminoethyl 6% crosslinked beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). This resin has a low content of aldehyde groups (15- 25 µm diaminoethyl / ml gel) providing a very good option to conjugate affinity ligands without distorting the target ligand. The backbone of the resin is 6% agarose beads and the pore size is adequate to work with enzymes & proteins mainly.
Aminoethyl resin give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.
Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.
Storage/Handling: Store at 4°C. Do not freeze.
IPTG, X-Gal and X-Gluc
©蚂蚁淘
没错!最开始,Gold Biotechnology只生产这三个产品,30年后的今天,Goldbio的产品已经超过3000种,涵盖:
琼脂糖树脂
抗生素
生化试剂
缓冲液
克隆与诱导
培养基添加剂
洗涤剂
DNA蛋白质电泳
酶、抑制剂和底物
生长因子
植物研究
蛋白质表达与纯化
还原剂……
目前Goldbio最核心的产品是:荧光素钾盐,X-gluc, 双丙氨磷,草胺磷 和 X-α-gal